Synthetic biotinylated tetra β(1→5) galactofuranoside for in vitro aspergillosis diagnosis. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Bioorganic and Medicinal Chemistry Année : 2011

Synthetic biotinylated tetra β(1→5) galactofuranoside for in vitro aspergillosis diagnosis.

Résumé

The synthesis of a tetra β(1→5) galactofuranoside was achieved using a thioglycoside donor with a methyl tert-butyl phenyl thio leaving group. This tetrasaccharide was conjugated to biotin and validated as antigen with the monoclonal antibody used for clinical detection of Aspergillus fumigatus galactomannan on streptavidin-coated microplates. Then we have shown its ability to detect antibodies associated with A. fumigatus induced disease by using sera from patients with Allergic broncho-pulmonary aspergillosis (ABPA) and correlated the results of antibody detection with those gained with a commercially available diagnostic test.

Domaines

Chimie organique

Dates et versions

hal-00616616 , version 1 (23-08-2011)

Identifiants

Citer

Laurent Cattiaux, Boualem Sendid, Mayeul Collot, Emeline Machez, Daniel Poulain, et al.. Synthetic biotinylated tetra β(1→5) galactofuranoside for in vitro aspergillosis diagnosis.. Bioorganic and Medicinal Chemistry, 2011, 19 (1), pp.547-55. ⟨10.1016/j.bmc.2010.10.062⟩. ⟨hal-00616616⟩
66 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More