Synthesis of cross-reactive carbohydrate determinants fragments as tools for in vitro allergy diagnosis. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Bioorganic and Medicinal Chemistry Année : 2011

Synthesis of cross-reactive carbohydrate determinants fragments as tools for in vitro allergy diagnosis.

Résumé

Four biotinylated tri and tetrasaccharide fragments of plant and invertebrate N-glycans were synthesized using methyl tert-butyl phenyl (MBP) thioglycosides donors in order to evaluate their involvement in cross-allergies as cross-reactive carbohydrate determinants (CCDs). Various levels of reactivity to anti-bee and anti-HRP antibodies and with sera from allergic patients were observed when the conjugates were coated on streptavidin microplates. The results showed the potential utility of these xylosylated and fucosylated oligosaccharide fragments in determining CCD antibody epitopes.

Domaines

Chimie organique

Dates et versions

hal-00616608 , version 1 (23-08-2011)

Identifiants

Citer

Mayeul Collot, Iain B H Wilson, Merima Bublin, Karin Hoffmann-Sommergruber, Jean-Maurice Mallet. Synthesis of cross-reactive carbohydrate determinants fragments as tools for in vitro allergy diagnosis.. Bioorganic and Medicinal Chemistry, 2011, 19 (3), pp.1306-20. ⟨10.1016/j.bmc.2010.12.001⟩. ⟨hal-00616608⟩
99 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More