The spread of plasmid-mediated quinolone resistance determinants (-like determinants) was evaluated in a collection of 232 ciprofloxacin-resistant or extended-spectrum β-lactamase (ESBL)-producing enterobacterial isolates recovered between November 2007 and May 2008 at Padua University Hospital, Italy. genes were mainly found in (68%) and to a lesser extent in (5.1%). Among the , and alleles found, the latter was by far the most frequent. Genetic environment analysis revealed that one gene in was embedded in an IS complex class 1 integron. All other genes were flanked by an IS region as part of the Tn transposon. and containing strains were not clonally related. Both topoisomerase II mutations and ESBL (mainly SHV-12, TEM-1 and TEM-150 types) were present in most of the -positive strains. is extremely frequent in isolates from Italy. In addition, association of with the IS mobile element in suggests a broad distribution of this resistance gene in the near future.