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Article Dans Une Revue Proteomics Année : 2009

A new generation of cross-linkers for selective detection by MALDI MS

Résumé

We designed a new cross-linker bearing a CHCA moiety. The use of the CHCA-tagged crosslinker JMV 3378 in conjunction with a neutral MALDI matrix a-cyano-4-hydroxycinnamic methyl ester enabled specific signal enhancement in MALDI-TOF MS of cross-link containing peptides. Discrimination between modified and non-modified peptides can be achieved by comparison of two spectra, one using CHCA and the other using the a-cyano-4- hydroxycinnamic methyl ester matrix. The methodology was validated using cytochrome c and apo-myoglobine as model proteins.

Dates et versions

hal-00580764 , version 1 (29-03-2011)

Identifiants

Citer

David Paramelle, Sonia Cantel, Christine Enjalbal, Muriel Amblard, Eric Forest, et al.. A new generation of cross-linkers for selective detection by MALDI MS. Proteomics, 2009, 9, pp.5384-5388. ⟨10.1002/pmic.200900562⟩. ⟨hal-00580764⟩
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