Prevalence and clonality of methicillin-resistant (MRSA) in the Atlantic Azores islands: predominance of SCC types IV, V and VI
Résumé
In order to obtain insights into the methicillin-resistant (MRSA) population structure in the Azores archipelago, 106 MRSA isolates were collected from patients attending an Azorean central hospital between January 2007 and February 2008. Antimicrobial resistance was determined for all isolates. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE), typing, multilocus sequence typing (MLST), staphylococcal chromosome cassette (SCC) typing and the presence of Panton–Valentine leukocidin (PVL). The majority of the isolates (87%, = 92) belonged to the EMRSA-15 clone (ST22, SCC-IVh), followed by the Pediatric clone (ST5-VI/IVc) (11%, = 12). The Berlin clone (ST45-IVa) and a new clone ( type t1839, ST1339 and SCC V variant) were represented by single isolates. All of the isolates carried SCC types IV, V or VI and a non-multiresistant antibiotic profile, resembling the currently emerging community MRSA. Moreover, PVL was described for the first time to be associated with the Pediatric clone carrying SCC type VI. We provided the first description of the population structure of MRSA in the Azores islands, which seems to be shaped by genetic events occurring locally, as well as by the regular population exchange between the islands, continental Portugal, the United Kingdom and the United States.
Origine : Fichiers produits par l'(les) auteur(s)
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