ANTIMICROBIAL PEPTIDES EXPRESSION BY OCULAR SURFACE CELLS IN RESPONSE TO ACANTHAMOEBA CASTELLANII: AN IN VITRO STUDY - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue British Journal of Ophthalmology Année : 2010

ANTIMICROBIAL PEPTIDES EXPRESSION BY OCULAR SURFACE CELLS IN RESPONSE TO ACANTHAMOEBA CASTELLANII: AN IN VITRO STUDY

Ahmad Muneer Otri
  • Fonction : Auteur
  • PersonId : 889652
Imran Mohammed
  • Fonction : Auteur
  • PersonId : 890984
Asiya Abedin
  • Fonction : Auteur
  • PersonId : 890985
Zhiyi Cao
  • Fonction : Auteur
  • PersonId : 890986
Andrew Hopkinson
  • Fonction : Auteur
  • PersonId : 890987

Résumé

Aims: Antimicrobial peptides (AMPs) are natural effectors of the innate immune response. Much work has been done to study their response and effects on bacterial and viral infection. Little if any information is available in relation to protozoal infections. Our aim was to comprehensively study the gene expression of the ocular AMPs in human corneal limbal epithelial cells (HCLE) stimulated with Acanthamoeba castellanii (AC). Methods: Human corneal limbal epithelial cells were exposed to AC at different time points, up to 9 hours, the genomic profile of the AMPs were analysed at these time point using real time PCR. HCLE cells not infected with AC were used as controls. Results: Seven of the 8 studied AMPs showed statistically significant upregulation in gene expression. Human beta Defensin 3 (hBD3) showed a very significant 10 fold upregulation in the exposed cells and Ribonuclease-7 (RNase-7) showed a very early and consistent increase. Human beta Defensin 1 (hBD1) was the only downregulated AMP. Conclusions: The study data suggests a possible role of the AMPs in combating the amoebic infection at the ocular surface. Using AMPs singly or in combination is a promising avenue for further exploration in the treatment of the sight threatening Acanthamoeba keratitis.

Mots clés

Fichier principal
Vignette du fichier
PEER_stage2_10.1136%2Fbjo.2009.178236.pdf (1.92 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-00561326 , version 1 (01-02-2011)

Identifiants

Citer

Ahmad Muneer Otri, Imran Mohammed, Asiya Abedin, Zhiyi Cao, Andrew Hopkinson, et al.. ANTIMICROBIAL PEPTIDES EXPRESSION BY OCULAR SURFACE CELLS IN RESPONSE TO ACANTHAMOEBA CASTELLANII: AN IN VITRO STUDY. British Journal of Ophthalmology, 2010, 94 (11), pp.1523. ⟨10.1136/bjo.2009.178236⟩. ⟨hal-00561326⟩

Collections

PEER
71 Consultations
225 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More