In vitro interference of β-lactams with biofilm development by prevalent community respiratory tract isolates
Résumé
Interference of cefditoren (CDN) and amoxicillin/clavulanic acid (AMC) with biofilm production was studied using 11 isolates with minimum inhibitory concentrations (MICs) ranging from 0.015μg/mL to 0.5μg/mL for CDN and from 0.06μg/mL to 2μg/mL for AMC (except for one isolate with an AMC MIC of 8μg/mL) and 5 isolates with MICs of 0.03–0.06μg/mL for CDN and 0.5–16μg/mL for AMC. Slime production was assessed in antibiotic-free medium and with 0.03μg/mL CDN or 1/0.5μg/mL AMC by measuring the optical density at 450nm (OD). Significantly lower mean OD values were obtained for with antibiotics compared with controls (CDN, 0.088 vs. 0.118, =0.003; and AMC, 0.095 vs. 0.112, =0.003), with significant correlation between both antibiotics (=0.752; =0.008). Percent reduction in OD values was higher for CDN compared with AMC (24.02% vs. 15.92%; =0.008). For , significantly lower mean OD values were obtained with CDN compared with controls (0.083 vs. 0.096; =0.043) but not with AMC (0.086 vs. 0.095; =0.08). Comparing percent reductions in versus for each antibiotic, no differences were found for AMC (15.92% vs. 9.40%; =0.36), with a tendency for CDN (24.02% vs. 13.79%; =0.069). Different β-lactams may have different capabilities of interfering with biofilm development when tested under the same experimental conditions.
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