Love wave biosensor for real-time detection of okadaic acid as DSP phycotoxin
Résumé
Love wave acoustic delay lines were used as sensors for okadaic acid inrmuno-detection through immobilized specific antibodies. Anti-okadaic acid antibodies were grafted onto the sensor surface. Antibodies, saturating agent, and target species, okadaic acid (test line) or an irrelevant peptide (control line), were injected through an optimized microfluidic chip. Similarly to sandwich ELISA, polyclonal antibodies were then injected a second time to bind to the acid. They led to frequency shifts three times superior with okadaic acid than with the peptide.