E6 and E7 proteins from different beta-papillomaviruses types do not interfere in UVB-induced apoptosis of HaCaT keratinocytes. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Experimental Dermatology Année : 2011

E6 and E7 proteins from different beta-papillomaviruses types do not interfere in UVB-induced apoptosis of HaCaT keratinocytes.

Résumé

Beta-papillomaviruses (beta-HPV) have been linked to the development of skin cancer in humans. Because both E6 and E7 proteins from beta-HPV have been involved in the potential carcinogenicity of these viruses, we investigated their role on UVB-induced apoptosis in HaCaT cell line. HaCaT cells have been transduced with both E6/E7 using a retroviral system and treated with PRIMA-1. Apoptosis was assessed by flow cytometry to measure mitochondrial membrane potential and DNA fragmentation. HaCat keratinocytes transduced with both E6 and E7 genes of seven beta-HPV types (HPV5, HPV8, HPV14, HPV24, HPV36, HPV38 and HPV49) did not demonstrate any inhibition of UVB-induced apoptosis, even after p53 reactivation through PRIMA-1. Our data suggest that the expression of E6 and E7 exert different modulatory effects on UVB-induced apoptosis according to beta-HPV types and to the cellular genetic context.

Domaines

Cancer

Dates et versions

hal-00553386 , version 1 (07-01-2011)

Identifiants

Citer

Jean-Sébastien Guerrini, Véronique Bouvard, Evelyne Oswald, Angel Alonso, Jean-Luc Prétet, et al.. E6 and E7 proteins from different beta-papillomaviruses types do not interfere in UVB-induced apoptosis of HaCaT keratinocytes.. Experimental Dermatology, 2011, 20 (1), pp.71-73. ⟨10.1111/j.1600-0625.2010.01197.x⟩. ⟨hal-00553386⟩

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