Communication Dans Un Congrès Année : 2008

Optical characterization of porous silicon microcavities for glucose oxidase biosensing - art. no. 69911Y

V. Agarwal
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D. B. Garcia
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Rene Legros
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Emile Perez
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Csilla Gergely

Résumé

PSi microcavity (PSiMc) is characterized by a narrow resonance peak in the optical spectrum that is very sensitive to small changes in the refractive index. We report that the resonant optical cavities of PSi structures can be used to enhance the detection of labeled fluorescent biomolecules. Various PSi configurations were tested in order to compare the optical response of the PSi devices to the capture of organic molecules. Morphological and topographical analyses were performed on PSiMc using Atomic Force (AFM) and Scanning Electron (SEM) microscopies. The heterogeneity in pores lengths resulting from etching process assures a better penetration of larger molecules into the pores and sensor sensitivity depends on the pore size. Molecular detection is monitored by the successive red shifts in the reflectance spectra after the stabilization of PSiMc with 3-aminopropyltriethoxysilane (APTES). The glucose oxidase was crosslinked into the PSiMc structures following a silane-glutaraldehyde (GTA) chemistry.

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Dates et versions

hal-00540392 , version 1 (26-11-2010)

Identifiants

  • HAL Id : hal-00540392 , version 1

Citer

Gabriela Palestino, V. Agarwal, D. B. Garcia, Rene Legros, Emile Perez, et al.. Optical characterization of porous silicon microcavities for glucose oxidase biosensing - art. no. 69911Y. Conference on Biophotonics - Photonic Solution for Better Health Care, Apr 2008, Strasbourg (FRANCE), France. pp.Y9911-Y9911. ⟨hal-00540392⟩
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