Hierarchization of Myogenic and Adipogenic Progenitors Within Human Skeletal Muscle. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Stem Cells / Stem Cells (Miamisburg) Année : 2010

Hierarchization of Myogenic and Adipogenic Progenitors Within Human Skeletal Muscle.

Résumé

Skeletal muscle cells constitute a heterogeneous population which maintains muscle integrity through a high myogenic regenerative capacity. More unexpectedly this population is also endowed with an adipogenic potential, even in humans, and intra-muscular adipocytes have been found to be present in several disorders. We tested the distribution of myogenic and adipogenic commitments in human muscle-derived cells in order to decipher the cellular basis of the myo-adipogenic balance. Clonal analysis showed that adipogenic progenitors can be separated from myogenic progenitors and, interestingly, from myo-adipogenic bipotent progenitors. These progenitors were isolated in the CD34(+) population on the basis of the expression of CD56 and CD15 cell surface markers. In vivo, these different cell types have been found in the interstitial compartment of human muscle. In vitro, we show that the proliferation of bipotent myo-adipogenic CD56(+)CD15(+) progenitors gives rise to myogenic CD56(+)CD15(-) progenitors and adipogenic CD56(-)CD15(+) progenitors. A cellular hierarchy of muscle and fat progenitors thus occurs within human muscle. These results provide cellular bases for adipogenic differentiation in human skeletal muscle, which may explain the fat development encountered in different muscle pathological situations.

Dates et versions

hal-00533750 , version 1 (08-11-2010)

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Citer

Didier F Pisani, Noémie Clement, Agnès Loubat, Magali Plaisant, Sabrina Sacconi, et al.. Hierarchization of Myogenic and Adipogenic Progenitors Within Human Skeletal Muscle.. Stem Cells / Stem Cells (Miamisburg), 2010, epub ahead of print. ⟨10.1002/stem.537⟩. ⟨hal-00533750⟩
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