Roles of PprA, IrrE, and RecA in the resistance of Deinococcus radiodurans to germicidal and environmentally relevant UV radiation. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Archiv für Mikrobiologie Année : 2009

Roles of PprA, IrrE, and RecA in the resistance of Deinococcus radiodurans to germicidal and environmentally relevant UV radiation.

Anja Bauermeister
  • Fonction : Auteur
Ralf Moeller
  • Fonction : Auteur
Petra Rettberg
  • Fonction : Auteur

Résumé

To study the role of different DNA repair genes in the resistance of Deinococcus radiodurans to mono- and polychromatic UV radiation, wild-type strain and knockout mutants in RecA, PprA, and IrrE of D. radiodurans were irradiated with UV-C (254 nm), UV-(A + B) (280-400 nm) and UV-A (315-400 nm) radiation, and survival was monitored. The strain deficient in recA was highly sensitive to UV-C radiation compared to the wild-type, but showed no loss of resistance against irradiation with UV-(A + B) and UV-A, while pprA and irrE-deficient strains exhibited elevated sensitivity to UV-A and UV-(A + B) radiation. These results suggest that the repair of DNA double-strand breaks is essential after treatment with highly energetic UV-C radiation, whereas protection from oxidative stress may play a greater role in resistance to environmentally relevant UV radiation.

Dates et versions

hal-00527410 , version 1 (19-10-2010)

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Citer

Anja Bauermeister, Esma Bentchikou, Ralf Moeller, Petra Rettberg. Roles of PprA, IrrE, and RecA in the resistance of Deinococcus radiodurans to germicidal and environmentally relevant UV radiation.. Archiv für Mikrobiologie, 2009, 191 (12), pp.913-8. ⟨10.1007/s00203-009-0522-7⟩. ⟨hal-00527410⟩
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