AgtA, the dicarboxylic amino acid transporter of Aspergillus nidulans, is concertedly down-regulated by exquisite sensitivity to nitrogen metabolite repression and ammonium-elicited endocytosis. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Eukaryotic Cell Année : 2009

AgtA, the dicarboxylic amino acid transporter of Aspergillus nidulans, is concertedly down-regulated by exquisite sensitivity to nitrogen metabolite repression and ammonium-elicited endocytosis.

Angeliki Apostolaki
  • Fonction : Auteur
Zoi Erpapazoglou
  • Fonction : Auteur
Laura Harispe
  • Fonction : Auteur
Maria Billini
  • Fonction : Auteur
Panagiota Kafasla
  • Fonction : Auteur
Dimosthenis Kizis
  • Fonction : Auteur
Miguel Angel Peñalva
  • Fonction : Auteur
Vicky Sophianopoulou
  • Fonction : Auteur

Résumé

We identified agtA, a gene that encodes the specific dicarboxylic amino acid transporter of Aspergillus nidulans. The deletion of the gene resulted in loss of utilization of aspartate as a nitrogen source and of aspartate uptake, while not completely abolishing glutamate utilization. Kinetic constants showed that AgtA is a high-affinity dicarboxylic amino acid transporter and are in agreement with those determined for a cognate transporter activity identified previously. The gene is extremely sensitive to nitrogen metabolite repression, depends on AreA for its expression, and is seemingly independent from specific induction. We showed that the localization of AgtA in the plasma membrane necessitates the ShrA protein and that an active process elicited by ammonium results in internalization and targeting of AgtA to the vacuole, followed by degradation. Thus, nitrogen metabolite repression and ammonium-promoted vacuolar degradation act in concert to downregulate dicarboxylic amino acid transport activity.

Dates et versions

hal-00527235 , version 1 (18-10-2010)

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Citer

Angeliki Apostolaki, Zoi Erpapazoglou, Laura Harispe, Maria Billini, Panagiota Kafasla, et al.. AgtA, the dicarboxylic amino acid transporter of Aspergillus nidulans, is concertedly down-regulated by exquisite sensitivity to nitrogen metabolite repression and ammonium-elicited endocytosis.. Eukaryotic Cell, 2009, 8 (3), pp.339-52. ⟨10.1128/EC.00270-08⟩. ⟨hal-00527235⟩
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