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Article Dans Une Revue FEMS Yeast Research Année : 2009

A phenotypic study of TFS1 mutants differentially altered in the inhibition of Ira2p or CPY

Aurélie Gombault
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Jonas Warringer
Robert Caesar
Béatrice Vallée
Helene Chautard
  • Fonction : Auteur
Anders Blomberg
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Hélène Bénédetti

Résumé

The Saccharomyces cerevisiae protein Tfs1p is known as a dual protein. On the one hand, it inhibits the carboxypeptidase Y protease, and on the other, it inhibits Ira2p, a GTPase-activating protein of Ras. We managed to dissect precise areas of Tfs1p specifically involved in only one of those functions. Based on these data, specific Tfs1p point mutants affected in only one of these two functions were constructed. In order to obtain insights on the physiological role of these functions, systematic phenotypic tests were performed on strains expressing these specific Tfs1p mutants. The results obtained demonstrate that the inhibition of Ira2p by Tfs1p is the predominant function under the conditions tested.

Dates et versions

hal-00522432 , version 1 (30-09-2010)

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Citer

Aurélie Gombault, Jonas Warringer, Robert Caesar, Fabienne Godin, Béatrice Vallée, et al.. A phenotypic study of TFS1 mutants differentially altered in the inhibition of Ira2p or CPY. FEMS Yeast Research, 2009, 9 (6), pp.867-874. ⟨10.1111/j.1567-1364.2009.00535.x⟩. ⟨hal-00522432⟩
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