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Article Dans Une Revue Analytical and Bioanalytical Chemistry Année : 2005

Comparison of the analysis of corticosteroids using different techniques.

Résumé

In this work we have optimized the analysis of 18 human corticosteroids, some endogenous (tetrahydrocortisol, tetrahydrocortisone, cortisol, and cortisone) and others synthetic (betamethasone, budesonide, cortisone acetate, desonide, dexamethasone, dexamethasone acetate, flunisolide, fluocinolone acetonide, halcinonide, methylprednisolone, prednisolone, prednisone, triamcinolone, and triamcinolone acetonide). Three analytical techniques were developed: ELISA, gas chromatography coupled with mass spectrometry (GC-MS), and liquid chromatography coupled with mass spectrometry (LC-MS). Several sample-preparation methods were optimized for each technique and enabled compounds of interest to be extracted from small urine samples (several mL). The results enabled us to assess the possibilities and the sensitivity of each technique for application to doping tests.

Dates et versions

hal-00509734 , version 1 (16-08-2010)

Identifiants

Citer

Estelle Pujos, Marie-Magdeleine Flament-Waton, Jean-Olivier Païssé, Marie-Florence Grenier-Loustalot. Comparison of the analysis of corticosteroids using different techniques.. Analytical and Bioanalytical Chemistry, 2005, 381 (1), pp.244-254. ⟨10.1007/s00216-004-2890-9⟩. ⟨hal-00509734⟩

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