Quantitative analysis of DHEA and androsterone in female urine: investigating the effects of menstrual cycle, oral contraception and training on exercise-induced changes in young women. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Analytical and Bioanalytical Chemistry Année : 2009

Quantitative analysis of DHEA and androsterone in female urine: investigating the effects of menstrual cycle, oral contraception and training on exercise-induced changes in young women.

Résumé

Dehydroepiandrosterone (DHEA) and its metabolite androsterone (A) are natural steroids secreted in high quantities in human body. To assess the influence of oral contraceptives, menstrual cycle phase, and also physical exercise (acute and chronic such as training) on these metabolites excretions, a collection of 28 female urine specimens was organized. A three-extraction-step method was developed, and the analyses were performed by gas chromatography-mass spectrometry using deuterated 19-noretiocholanolone as the internal standard. Sample hydration state was found to be of great importance for kinetic studies, as it directly influenced the concentrations. No influence of menstrual cycle and training was found for androsterone and DHEA. However, oral contraceptive intake lowered DHEA excretion in urine and A seems to be slightly affected by exercise.

Dates et versions

hal-00509309 , version 1 (11-08-2010)

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Marie-Laure Bayle, C. Enea, Patrick Goetinck, Florent Lafay, Nathalie Boisseau, et al.. Quantitative analysis of DHEA and androsterone in female urine: investigating the effects of menstrual cycle, oral contraception and training on exercise-induced changes in young women.. Analytical and Bioanalytical Chemistry, 2009, 393 (4), pp.1315-25. ⟨10.1007/s00216-008-2549-z⟩. ⟨hal-00509309⟩
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