Contribution of microextraction in packed sorbent for the analysis of cotinine in human urine by GC-MS.
Résumé
A simple, rapid, sensitive, and non-consuming solvent method for the determination of cotinine in urine was developed, based on sample preparation by the relatively new technique microextraction in packed sorbent (MEPS) and analysis by GC-MS. This optimized method was compared with conventional solid-phase extraction/liquid-liquid extraction method used as reference. The wide linear range (5-5,000 ng/mL) and high sensitivity of the MEPS method (limit of detection 0.8 ng/mL) allow application to analysis of urine from smokers as well as non-smokers susceptible to passive smoking.