Cross-presentation by dendritic cells from live cells induces protective immune responses in vivo. - Archive ouverte HAL Access content directly
Journal Articles Blood Year : 2010

Cross-presentation by dendritic cells from live cells induces protective immune responses in vivo.

Diana Matheoud
  • Function : Author
Leila Perié
Guillaume Hoeffel
Lene Vimeux
  • Function : Author
Isabelle Parent
  • Function : Author
Laurent Renia
  • Function : Author
Bruno Lucas
Vincent Feuillet
  • Function : Author
Anne Hosmalin

Abstract

Cross-presentation is an essential mechanism that allows dendritic cells (DCs) to efficiently present exogenous antigens to CD8(+) T cells. Among cellular antigen sources, apoptotic cells are commonly considered as the best for cross-presentation by DCs. However, the potential of live cells as a source of antigen has been overlooked. Here we explored whether DCs were able to capture and cross-present antigens from live cells. DCs internalized cytosolic and membrane material into vesicles from metabolically labeled live cells. Using time-lapse confocal microscopy in whole spleens, we showed that DCs internalized material from live cells in vivo. After ovalbumin uptake from live cells, DCs cross-primed ovalbumin-specific naive OT-I CD8(+) T cells in vitro. Injected into mice previously transferred with naive OT-I T cells, they also cross-primed in vivo, even in the absence of endogenous DCs able to present the epitope in the recipient mice. Interestingly, DCs induced stronger natural CD8(+) T-cell responses and protection against a lethal tumor challenge after capture of antigens from live melanoma cells than from apoptotic melanoma cells. The potential for cross-presentation from live cells uncovers a new type of cellular intercommunication and must be taken into account for induction of tolerance or immunity against self, tumors, grafts, or pathogens.
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Dates and versions

hal-00493469 , version 1 (19-06-2010)

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Diana Matheoud, Leila Perié, Guillaume Hoeffel, Lene Vimeux, Isabelle Parent, et al.. Cross-presentation by dendritic cells from live cells induces protective immune responses in vivo.. Blood, 2010, 115 (22), pp.4412-20. ⟨10.1182/blood-2009-11-255935⟩. ⟨hal-00493469⟩

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