Adenovirus and mycoplasma infection in an ornate box turtle () in Hungary
Résumé
A female, adult ornate box turtle () with fatty liver was submitted for virologic examination in Hungary. Signs of an adenovirus infection including degeneration of the liver cells, enlarged nuclei and intranuclear inclusion bodies were detected by light microscopic examination. The presence of an adenovirus was later confirmed by obtaining partial sequence data from the adenoviral DNA-dependent DNA-polymerase. Phylogenetic analyses revealed that this novel chelonian adenovirus was distinct from previously described reptilian adenoviruses, not belonging to any of the recognized genera of the family . As a part of the routine diagnostic procedure for chelonians the detection of herpes-, rana- and iridoviruses together with spp. was attempted. Amplicons were generated by a general mycoplasma polymerase chain reaction (PCR) targeting the 16S/23S ribosomal RNA (rRNA) intergenic spacer region, as well as, a specific PCR targeting the 16S rRNA gene. Based on the analyses of partial sequences of the 16S rRNA gene, the sp. of the ornate box turtle seemed to be identical with the recently described eastern box turtle () sp. This is the first report of a novel chelonian adenovirus and a mycoplasma infection in an ornate box turtle () in Europe.
Origine : Fichiers produits par l'(les) auteur(s)
Loading...