Three-dimensional structure of recombinant type 1 inositol 1,4,5-trisphosphate receptor - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical Journal Année : 2010

Three-dimensional structure of recombinant type 1 inositol 1,4,5-trisphosphate receptor

Francis Wolfram
  • Fonction : Auteur
Edward Morris
  • Fonction : Auteur
Colin W Taylor
  • Fonction : Auteur correspondant
  • PersonId : 870296

Connectez-vous pour contacter l'auteur

Résumé

Inositol 1,4,5-trisphosphate receptors (IP3R) are the intracellular channels that mediate release of Ca2+ from the endoplasmic reticulum in response to the many stimuli that evoke IP3 formation. We characterized and purified type 1 IP3R heterologously expressed in Sf9 cells, and used the purified IP3R1 to determine its three-dimensional structure by electron microscopy and single particle analysis. Recombinant IP3R1 has four-fold symmetry with overall dimensions of about 19.5 x 19.5 x 17.5 nm3. It comprises a small domain, which is likely to include the pore, linked by slender bridges to a large cytoplasmic domain with four petal-like regions. Our structures of recombinant IP3R1 and native cerebellar IP3R have similar appearances and dimensions. The only notable difference is the absence of a central stigma-like domain from the cytoplasmic region of recombinant IP3R1. The first structure of a recombinant IP3R is an important step towards developing 3D structures of IP3R that better contribute to understanding the structural basis of IP3R activation.

Mots clés

Fichier principal
Vignette du fichier
PEER_stage2_10.1042%2FBJ20100143.pdf (1.02 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-00486861 , version 1 (27-05-2010)

Identifiants

Citer

Francis Wolfram, Edward Morris, Colin W Taylor. Three-dimensional structure of recombinant type 1 inositol 1,4,5-trisphosphate receptor. Biochemical Journal, 2010, 428 (3), pp.483-489. ⟨10.1042/BJ20100143⟩. ⟨hal-00486861⟩

Collections

PEER
45 Consultations
65 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More