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Article Dans Une Revue Cell Calcium Année : 2010

Effects of elevated intracellular calcium on the osmotic fragility of human red blood cells.

Anne Cueff
  • Fonction : Auteur
Rachel Seear
  • Fonction : Auteur
Agnieszka Dyrda
  • Fonction : Auteur
Guillaume Bouyer
Stéphane Egée
  • Fonction : Auteur
Alessandro Esposito
  • Fonction : Auteur
Jeremy Skepper
  • Fonction : Auteur
Teresa Tiffert
  • Fonction : Auteur
Virgilio L Lew
  • Fonction : Auteur
Serge L y Thomas
  • Fonction : Auteur

Résumé

High throughput methodologies that measure the distribution of osmotic fragilities in red blood cell populations have enabled the investigation of dynamic changes in red cell homeostasis and membrane permeability in health and disease. The common assumption in the interpretation of dynamic changes in osmotic fragility curves is that left or right shifts reflect a decreased or increased hydration state of the cells, respectively, allowing direct inferences on membrane transport from osmotic fragility measurements. However, the assumed correlation between shifts in osmotic fragility and hydration state has never been directly explored, and may prove invalid in certain conditions. We investigated here whether this correlation holds for red cells exposed to elevated intracellular calcium. The results showed that elevated cell calcium causes a progressive increase in osmotic fragility with minimal contribution from cell hydration (<8%). Loss of membrane area by the release of 160+/-40nm diameter (mean+/-SD) vesicles is shown to be a major contributor, but may not account for the full non-hydration component. The rest must reflect a specific calcium-induced lytic vulnerability of the membrane causing rupture before the cells attain their maximal spherical volumes. The implications of these findings are discussed.

Dates et versions

hal-00486424 , version 1 (25-05-2010)

Identifiants

Citer

Anne Cueff, Rachel Seear, Agnieszka Dyrda, Guillaume Bouyer, Stéphane Egée, et al.. Effects of elevated intracellular calcium on the osmotic fragility of human red blood cells.. Cell Calcium, 2010, 47 (1), pp.29-36. ⟨10.1016/j.ceca.2009.11.002⟩. ⟨hal-00486424⟩
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