Primary and three-dimensional structure of lactotransferrin (lactoferrin) glycans. - Archive ouverte HAL Access content directly
Journal Articles Advances in Experimental Medicine and Biology Year : 1994

Primary and three-dimensional structure of lactotransferrin (lactoferrin) glycans.

Abstract

In order to establish relationships between glycan structure and biological activity, the authors undertook a comparative study of the glycan primary structure of different transferrins from several species. By associating permethylation-mass spectrometry and 1H-NMR spectroscopy, the primary structure of the human, bovine, caprine, murine and porcine lactotransferrin glycans were determined. Using the same methods, the glycan structure of 9 serotransferrins was determined. The results obtained led to the conclusion that glycans are specific for each transferrin and, for a given transferrin, specific to the species. No relationship could be established between primary structure and function of transferrin glycans. Glycan molecular modelling, molecular dynamics simulations and X-ray diffraction studies of free glycans confirm the mobility in space of antennae. In contrast, the glycan associated with a protein is immobilized into only one conformation, as in the case of glycan-lectin associations or of "internal" glycan-protein interactions, like in rabbit serotransferrin, in which the glycan forms a bridge between the two lobes of the peptide chain, and maintains the protein in a biologically active conformation. In the case of human sero- and lactotransferrins, the glycans are in an external position on the molecules and could play a role of recognition signals.
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Dates and versions

hal-00484469 , version 1 (18-05-2010)

Identifiers

  • HAL Id : hal-00484469 , version 1
  • PUBMED : 7762433

Cite

G. Spik, B. Coddeville, J. Mazurier, Yves Bourne, C. Cambillaut, et al.. Primary and three-dimensional structure of lactotransferrin (lactoferrin) glycans.. Advances in Experimental Medicine and Biology, 1994, 357, pp.21-32. ⟨hal-00484469⟩

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