Format du dépôt |
Notice |
Type de dépôt |
Article dans une revue |
Titre |
en
Design of antibody-reactive peptides from discontinuous parts of scorpion toxins.
|
Résumé |
en
The Amm VIII protein was previously isolated from the venom of the scorpion Androctonus mauretanicus mauretanicus. Despite 87% identity with AaH II, the most toxic alpha-type scorpion toxin, Amm VIII is not toxic to mice. However, antisera against Amm VIII protect mice from AaH II lethal action. Here, we report that the Amm VIII protein elicits antibodies that only recognize discontinuous-type epitopes since we could not observe any antibody binding to overlapping 12-mer peptides covering the whole Amm VIII sequence. By using a new bioinformatic tool, 24 peptides mimicking discontinuous regions of Amm VIII were designed in silico, then prepared by Spot synthesis. Seven of these discontinuous-continuous peptides were recognized by anti-Amm VIII antibodies. Analysis of the 3D location of the segments that compose the antigenically reactive discontinuous-continuous peptides, allowed us to group those antigenic segments into three regions of Amm VIII, putatively corresponding to discontinuous antigenic regions of alpha-type scorpion toxins. Anti-Amm VIII antibodies were also found to cross-react towards several of the discontinuous-continuous peptides designed from the AaH II structure, pointing to a possible involvement of the corresponding discontinuous epitopes in the capacity displayed by anti-Amm VIII antibodies to neutralize AaH II. Altogether, our results show that it is possible to design antibody-reactive peptides from discontinuous parts of scorpion toxins. The position of the reactive segments in the structural context of scorpion toxins highlights the antigenic properties of the Amm VIII anatoxin and concurs to explain the capacity of anti-Amm VIII antibodies to neutralize the potent AaH II toxin.
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Auteur(s)
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L. Alvarenga
, V. Moreau
1
, L. Felicori
, Cong Tu Nguyen
2
, C. Duarte
, C. Chavez-Olortegui
, F. Molina
, M.-F. Martin-Eauclaire
3
, C. Granier
4
1
EDF -
EDF
( 302313 )
- France
2
TCEM -
Transfert Sol-Plante et Cycle des Eléments Minéraux dans les Ecosystèmes Cultivés
( 37934 )
- Centre de Recherche INRA Bordeaux Aquitaine - 71, Avenue Edouard Bourlaux - BP 81, 33883 Villenave d’ornon Cedex France
- France
-
Institut National de la Recherche Agronomique UR1220 ( 92114 )
;
-
École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux ( 300411 )
3
CRN2M -
Centre de recherche en neurobiologie - neurophysiologie de Marseille
( 192792 )
- Faculté de Médecine
Secteur Nord
Boulevard Pierre Dramard
13916 MARSEILLE CEDEX 20
- France
-
Aix Marseille Université UMR7286 ( 198056 )
;
-
Institut National de la Santé et de la Recherche Médicale UMR7286 ( 303623 )
;
-
Centre National de la Recherche Scientifique UMR7286 ( 441569 )
4
IBCP -
Institut de biologie et chimie des protéines [Lyon]
( 581 )
- 7 Passage du Vercors 69367 LYON CEDEX 07
- France
-
Université Claude Bernard Lyon 1 ( 194495 )
;
-
Université de Lyon ( 301088 )
;
-
Centre National de la Recherche Scientifique UMS3760 / FR3302 ( 441569 )
|
Comité de lecture |
Oui
|
Vulgarisation |
Non
|
Langue du document |
Anglais
|
Nom de la revue |
|
Audience |
Internationale
|
Date de publication |
2010-01-22
|
Date de publication électronique |
2009-12-03
|
Volume |
28
|
Numéro |
4
|
Page/Identifiant |
970-80
|
Public visé |
Scientifique
|
Domaine(s) |
-
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]
|
Voir aussi |
-
http://hal.archives-ouvertes.fr/hal-00482304
-
http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&term=19962461
|
Mots-clés (Mesh) |
-
Amino Acid Sequence
-
Animals
-
Molecular Sequence Data
-
Neutralization Tests
-
Protein Structure, Tertiary
-
Rabbits
-
Scorpion Venoms
-
Scorpions
-
Antibodies, Neutralizing
-
Antitoxins
-
Cross Reactions
-
Epitope Mapping
-
Epitopes
-
Mice
-
Mice, Inbred C57BL
-
Models, Molecular
|
Mots-clés |
en
Amino Acid Sequence, Animals, Antibodies, Neutralizing, Antitoxins, Cross Reactions, Epitope Mapping, Epitopes, Mice, Inbred C57BL, Molecular Sequence Data, Neutralization Tests, Tertiary, Rabbits, Scorpion Venoms, Scorpions
fr
Models, Molecular, Protein Structure
|
DOI |
10.1016/j.vaccine.2009.10.135 |
ProdINRA |
248103 |
Pubmed Id |
19962461 |
UT key WOS |
000274943300020 |