Isolation, crystallization and preliminary X-ray analysis of the transamidosome, a ribonucleoprotein involved in asparagine formation. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Acta crystallographica Section F : Structural biology communications [2014-...] Année : 2009

Isolation, crystallization and preliminary X-ray analysis of the transamidosome, a ribonucleoprotein involved in asparagine formation.

Marc Bailly
  • Fonction : Auteur
Mickael Blaise
Soren Thirup
  • Fonction : Auteur

Résumé

Thermus thermophilus deprived of asparagine synthetase synthesizes Asn on tRNA(Asn) via a tRNA-dependent pathway involving a nondiscriminating aspartyl-tRNA synthetase that charges Asp onto tRNA(Asn) prior to conversion of the Asp to Asn by GatCAB, a tRNA-dependent amidotransferase. This pathway also constitutes the route of Asn-tRNA(Asn) formation by bacteria and archaea deprived of asparaginyl-tRNA synthetase. The partners involved in tRNA-dependent Asn formation in T. thermophilus assemble into a ternary complex called the transamidosome. This particule produces Asn-tRNA(Asn) in the presence of free Asp, ATP and an amido-group donor. Crystals of the transamidosome from T. thermophilus were obtained in the presence of PEG 4000 in MES-NaOH buffer pH 6.5. They belonged to the primitive monoclinic space group P2(1), with unit-cell parameters a = 115.9, b = 214.0, c = 127.8 A, beta = 93.3 degrees . A complete data set was collected to 3 A resolution. Here, the isolation and crystallization of the transamidosome from T. thermophilus and preliminary crystallographic data are reported.

Dates et versions

hal-00477259 , version 1 (28-04-2010)

Identifiants

Citer

Marc Bailly, Mickael Blaise, Bernard Lorber, Soren Thirup, Daniel Kern. Isolation, crystallization and preliminary X-ray analysis of the transamidosome, a ribonucleoprotein involved in asparagine formation.. Acta crystallographica Section F : Structural biology communications [2014-..], 2009, 65 (Pt 6), pp.577-81. ⟨10.1107/S1744309109015000⟩. ⟨hal-00477259⟩

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