Despite the high complexity and variability of estuaries, these ecosystems are very productive and play an important role in fish feeding. This paper constitutes a preliminary investigation to test how fish optimize the use of the available trophic resources, by studying trophic preference variability and feeding strategies of some pelagic and demersal fish in the Gironde estuary (southwest France). Fish and their prey were collected approximately every two months from July 2003 to June 2004 in the upstream area of the saline estuary. Stomach content analyses were realized to describe the variability of fish feeding according to their size and the time of year. Intra- and interspecific food niche overlap was evaluated using Schoener's index and a cross-calculation method was used to highlight the general fish trends in predation strategy. Stomach content results showed interspecific and intraspecific variability in fish feeding, which can be explained by their different or evolutionary ecomorphology. Their diets are composed mainly of zooplankton and hyperbenthic crustaceans with temporal variations in the consumed taxa. Optimization of the available trophic resource use, a key element in estuarine resilience, is thus possible due to the temporal adaptation of this structural trophic web. However, in spite of their temporal adaptation capacity, most fish species exhibited a specialist feeding strategy. This result was not expected, especially in the high turbidity of the Gironde estuary; the loss of one of these species could affect the fish trophic web structure and hence the resilience of the system.