Interaction between the C-terminal domains of N and P proteins of measles virus investigated by NMR. - Archive ouverte HAL Access content directly
Journal Articles FEBS Letters Year : 2009

Interaction between the C-terminal domains of N and P proteins of measles virus investigated by NMR.

Abstract

In this paper we investigate the interaction between the C-terminal domains of the measles virus phosphoprotein (XD) and nucleoprotein (N(TAIL)) by using nuclear magnetic resonance chemical shift perturbation experiments. Using both N(TAIL) constructs and peptides, we show that contrary to the conserved Box2 region (N(489-506)), the C-terminal region of N(TAIL) (N(513-525)) does not directly interact with XD, and yet affects binding to XD. We tentatively propose a model where the C-terminus of N(TAIL) would stabilize the N(TAIL)-XD complex either via a functional coupling with N(489-506) or by reducing the entropic penalty associated to the binding-coupled-to-folding process.

Dates and versions

hal-00473784 , version 1 (16-04-2010)

Identifiers

Cite

Cedric Bernard, Stéphane Gely, Jean-Marie Bourhis, Xavier Morelli, Sonia Longhi, et al.. Interaction between the C-terminal domains of N and P proteins of measles virus investigated by NMR.. FEBS Letters, 2009, 583 (7), pp.1084-9. ⟨10.1016/j.febslet.2009.03.004⟩. ⟨hal-00473784⟩
332 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More