Structure of electron-capture dissociation fragments from charge-tagged peptides probed by tunable infrared multiple photon dissociation. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of the American Chemical Society Année : 2008

Structure of electron-capture dissociation fragments from charge-tagged peptides probed by tunable infrared multiple photon dissociation.

Résumé

In this study, we propose the first spectroscopic structural characterization of c-type ions produced by ECD of a peptide. The structure of c-type ions formed by electron capture dissociation and the overall mechanism leading to their formation are still a question of debate. Depending on the mechanism, c-type ions have been proposed to have either an enol-imine structure (-C(OH)NH) or an amide one (-C(O)-NH2). Since these ions are isomeric, mass spectrometry only cannot discriminate between them, but infrared spectroscopy can bring experimental evidence and help determine which scheme is operative. Using the coupling between a tunable free electron laser and a FT-ICR mass spectrometer, we show that c-type ions have an amide structure, characterized by an IR signature of the C=O stretch at 1731 cm(-1). This result is particularly interesting from the perspective of the elucidation of the ECD mechanism.

Dates et versions

hal-00467063 , version 1 (25-03-2010)

Identifiants

Citer

Gilles Frison, Guillaume van Der Rest, Frantisek Turecek, Thierry Besson, Joël Lemaire, et al.. Structure of electron-capture dissociation fragments from charge-tagged peptides probed by tunable infrared multiple photon dissociation.. Journal of the American Chemical Society, 2008, 130 (45), pp.14916-14917. ⟨10.1021/ja805257v⟩. ⟨hal-00467063⟩
223 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More