Fluorogenic substrates for the screening assay of transketolase through beta-elimination of umbelliferone-development, scope and limitations? - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biotechnology Année : 2009

Fluorogenic substrates for the screening assay of transketolase through beta-elimination of umbelliferone-development, scope and limitations?

Résumé

5-O-Coumarinyl-d-xylulose was studied as a fluorogenic substrate for the stereospecific assay of transketolase enzyme. Enzymatic C2–C3 cleavage released an α-hydroxyl, β-coumarinyl substituted aldehyde. Although the subsequent β-elimination step was rate limiting under chemical or enzymatic catalysis, we detected a TK activity as low as 0.7 mIU. To improve the fluorescence signal release, kinetic and product distribution analyses of this reaction were performed by LC/UV/MS coupling.
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hal-00455188 , version 1 (09-02-2010)

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  • HAL Id : hal-00455188 , version 1

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Franck Charmantray, Bertrand Legeret, Virgil Hélaine, Laurence Hecquet. Fluorogenic substrates for the screening assay of transketolase through beta-elimination of umbelliferone-development, scope and limitations?. Journal of Biotechnology, 2009, 145, pp.359-366. ⟨hal-00455188⟩
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