High-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples - Archive ouverte HAL Access content directly
Journal Articles Optics Letters Year : 2009

High-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples

Abstract

We have developed a tomographic diffractive microscope, equipped with a fluorescence confocal scanner. We measure experimentally the lateral resolution using an edge method and by comparing tomographic images of the same samples with wide-field and laser scanning confocal microscopy images; a scanning electron microscope image serves as a reference. The experimental resolution is shown to be to about 130 nm, or / 3.5 NA . This instrument also permits one to measure 3D, complex index of refraction distributions, a quantity that is not accessible to conventional microscopes, and we show how this feature may be used to observe KCl crystals, absorption of which is very weak.
Fichier principal
Vignette du fichier
MIPS_2009_07_ARTICLE.pdf (262.69 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-00453711 , version 1 (03-10-2013)

Identifiers

Cite

Matthieu Debailleul, Vincent Georges, Bertrand Simon, R. Morin, Olivier Haeberlé. High-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples. Optics Letters, 2009, 34 (1), pp.79-81. ⟨10.1364/OL.34.000079⟩. ⟨hal-00453711⟩
109 View
314 Download

Altmetric

Share

Gmail Facebook X LinkedIn More