A nuclear juvenile hormone-binding protein from larvae of Manduca sexta: a putative receptor for the metamorphic action of juvenile hormone - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 1994

A nuclear juvenile hormone-binding protein from larvae of Manduca sexta: a putative receptor for the metamorphic action of juvenile hormone

S. R. Palli
  • Fonction : Auteur
K. Touhara
  • Fonction : Auteur
B. C. Bonning
  • Fonction : Auteur
J. Atkinson
  • Fonction : Auteur
S. C. Trowell
  • Fonction : Auteur
Kiyoshi Hiruma
  • Fonction : Auteur
W. G. Goodman
  • Fonction : Auteur
T. Kyriakides
  • Fonction : Auteur
Glen D. Prestwich
  • Fonction : Auteur

Résumé

A 29-kDa nuclear juvenile hormone (JH)-binding protein from the epidermis of Manduca sexta larvae was purified by using the photoaffinity analog for JH II ([3H]epoxyhomofarnesyldiazoacetate) and partially sequenced. A 1.1-kb cDNA was isolated by using degenerate oligonucleotide primers for PCR based on these sequences. The cDNA encoded a 262-amino acid protein that showed no similarity with other known proteins, except for short stretches of the interphotoreceptor retinoid-binding protein, rhodopsin, and human nuclear protein p68. Recombinant baculovirus containing this cDNA made a 29-kDa protein that was covalently modified by [3H]epoxyhomofarnesyldiazoacetate and specifically bound the natural enantiomer of JH I (Kd = 10.7 nM). This binding was inhibited by the natural JHs but not by methoprene. Immunocytochemical analysis showed localization of this 29-kDa protein to epidermal nuclei. Both mRNA and protein are present during the intermolt periods; during the larval molt, the mRNA disappears but the protein persists. Later when cells become pupally committed, both the mRNA and protein disappear with a transient reappearance near pupal ecdysis. The properties of this protein are consistent with its being the receptor necessary for the antimetamorphic effects of JH.
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Dates et versions

hal-00451554 , version 1 (29-01-2010)

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  • HAL Id : hal-00451554 , version 1

Citer

S. R. Palli, K. Touhara, Jean-Philippe Charles, B. C. Bonning, J. Atkinson, et al.. A nuclear juvenile hormone-binding protein from larvae of Manduca sexta: a putative receptor for the metamorphic action of juvenile hormone. Proceedings of the National Academy of Sciences of the United States of America, 1994, 91 (13), pp.6191-5. ⟨hal-00451554⟩
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