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Article Dans Une Revue Nanotechnology Année : 2008

How nanoparticles encapsulating fluorophores allow a double detection of biomolecules by localized surface plasmon resonance and luminescence

Résumé

The paper shows how polysiloxane particles encapsulating fluorophores can be successfully used to detect biotin-streptavidin binding by two types of technique. After functionalization of the particles by streptavidin, the fixation of the biomolecule can indeed be detected by a shift of the localized surface plasmon resonance of the biotinylated gold dots used as substrate and by the luminescence of the fluorophores evidenced by scanning near-field optical microscopy. The development of particles allowing such a double detection opens a route for increasing the reliability of biological detection and for multi-labelling strategies crossing both detection principles.

Dates et versions

hal-00433994 , version 1 (20-11-2009)

Identifiants

Citer

Grégory Barbillon, Anne Charlotte Faure, Nayla El Kork, Pascal Moretti, Stéphane Roux, et al.. How nanoparticles encapsulating fluorophores allow a double detection of biomolecules by localized surface plasmon resonance and luminescence. Nanotechnology, 2008, pp.1. ⟨10.1088/0957-4484/19/03/035705⟩. ⟨hal-00433994⟩
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