Comparison of inert supports in laser desorption/ionization mass spectrometry of peptides ; pencil lead, porous silica gel, DIOS-chip and NALDITM target - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Rapid Communications in Mass Spectrometry Année : 2009

Comparison of inert supports in laser desorption/ionization mass spectrometry of peptides ; pencil lead, porous silica gel, DIOS-chip and NALDITM target

Résumé

In the search for alternative inert surfaces replacing silicon chips in Desorption/Ionization On porous Silicon (DIOS)-like mass spectrometry analyses, nanostructured silicon-based NALDITM chips were evaluated in Laser Desorption/ionization (LDI) of peptides. Comparisons were made using commercially available DIOS chips (MassPREPTM-DIOS-targetTM), amorphous carbon powder from lead pencil and porous silica gel used for chromatographic purposes as reference supports. A set of synthetic model peptides presenting variable amino acid sequences of various lenghts was analyzed under all conditions. The LDI responses of the four 'matrix-free' techniques were compared, especially in terms of peptide detection sensitivity and overall experiment robustness.

Domaines

Chimie organique

Dates et versions

hal-00432904 , version 1 (17-11-2009)

Identifiants

Citer

Nawar Shenar, Sonia Cantel, Jean Martinez, Christine Enjalbal. Comparison of inert supports in laser desorption/ionization mass spectrometry of peptides ; pencil lead, porous silica gel, DIOS-chip and NALDITM target. Rapid Communications in Mass Spectrometry, 2009, 23, pp.2371-2379. ⟨10.1002/rcm.4158⟩. ⟨hal-00432904⟩
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