Presence of rhamnogalacturonan II in the juices produced by enzymatic liquefaction of Agave pulquero stem (Agave mapisaga) - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Carbohydrate Polymers Année : 2009

Presence of rhamnogalacturonan II in the juices produced by enzymatic liquefaction of Agave pulquero stem (Agave mapisaga)

Résumé

Rhamnogalacturonan II (RG-II), a small complex pectic polysaccharide is released from Agave pulquero stems (Agave mapisaga), after the production period of aguamiel. RG-II was obtained by treatment with two commercial liquefying enzyme preparations, it was isolated by size-exclusion chromatography and characterized. RG-IIs contains diagnostic sugars such as apiose, 2-O-methyl-L-fucose, 2-O-methyl-D-xylose, aceric acid, Kdo and Dha. Glycosyl-linkage compositions of the Agave pulquero RG-II like structures are similar to the theoretical model described through sycamore RG-II structure. The presence of T-linked apiose indicates that the obtained juice from Agave pulquero plant contains the free RG-II dimer. Thus, when pectinolytic enzyme preparations are used to process Agave pulquero (A. mapisaga), RG-II is released as one of the main soluble polysaccharide fraction.

Dates et versions

hal-00424587 , version 1 (16-10-2009)

Identifiants

Citer

Ri. Ortiz-Basurto, Pascale Williams, M-P. Belleville, Thierry Doco. Presence of rhamnogalacturonan II in the juices produced by enzymatic liquefaction of Agave pulquero stem (Agave mapisaga). Carbohydrate Polymers, 2009, 77 (4), pp.870 - 875. ⟨10.1016/j.carbpol.2009.03.001⟩. ⟨hal-00424587⟩
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