A new immunosensor for breast cancer cell detection using antibody-coated long alkylsilane self-assembled monolayers in a parallel plate flow chamber.
Jean-Claude Ehrhart
,
Bernard Bennetau
(1)
,
Louis Renaud
(2)
,
Jean-Pierre Madrange
,
Laurent Thomas
(3)
,
Julie Morisot
(4)
,
Arnaud Brosseau
(5)
,
Sylvain Allano
,
Patrick Tauc
(6)
,
Phuong-Lan Tran
(4)
1
ISM -
Institut des Sciences Moléculaires
2 CERCO - Centre de recherche cerveau et cognition
3 Department of Physics
4 SATIE - Systèmes et Applications des Technologies de l'Information et de l'Energie
5 PPSM - Laboratoire de Photophysique et Photochimie Supramoléculaires et Macromoléculaires
6 LBPA - Laboratoire de Biologie et de Pharmacologie Appliquée
2 CERCO - Centre de recherche cerveau et cognition
3 Department of Physics
4 SATIE - Systèmes et Applications des Technologies de l'Information et de l'Energie
5 PPSM - Laboratoire de Photophysique et Photochimie Supramoléculaires et Macromoléculaires
6 LBPA - Laboratoire de Biologie et de Pharmacologie Appliquée
Jean-Claude Ehrhart
- Fonction : Auteur
Louis Renaud
- Fonction : Auteur
- PersonId : 171618
- IdHAL : louis-renaud
- ORCID : 0000-0002-5762-7715
- IdRef : 085267848
Jean-Pierre Madrange
- Fonction : Auteur
Sylvain Allano
- Fonction : Auteur
Patrick Tauc
- Fonction : Auteur
- PersonId : 846283
- IdRef : 033275955
Résumé
We designed a new efficient and reliable immunosensor and demonstrated its analytic performance to capture breast cancer MCF7 and T47D cells, under laminar flow, onto antibody-coated long alkylsilane self-assembled monolayers (SAMs) in a parallel plate flow chamber. The surface floor of the laminar flow chamber was grafted with an amino-terminated long alkyl chain spacer, 21-aminohenicosyl trichlorosilane (AHTS) followed by tethering a specific monoclonal antibody directed against the human epithelial cell adhesion molecule (EpCAM) antigen, which is overexpressed in primary breast cancer. Properties of the AHTS- and antibody-grafted surface floor were compared to that of surface floors coated with the short alkyl spacers 3-glycidoxy-propyl trimethoxysilane (GPTS) or 3-aminopropyl triethoxysilane (APTES) and antibodies. A theoretical model was constructed according to the geometry of the flow chamber in order to calculate the trajectories that would use cell flows. Cell capture experiments demonstrated that cell immobilization was optimized throughout the whole flow chamber. High cell capture was yielded on antibody-tethered long alkyl AHTS surface. This new procedure offers multiple advantages: a versatile tool readily applied to a panel of purified antibodies, an enrichment of cell immobilization using repetitive cell flow, and a stable capturing surface suitable for long term storage and handling.
Domaines
BiophysiqueFormat du dépôt | Notice |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
A new immunosensor for breast cancer cell detection using antibody-coated long alkylsilane self-assembled monolayers in a parallel plate flow chamber.
|
Résumé |
en
We designed a new efficient and reliable immunosensor and demonstrated its analytic performance to capture breast cancer MCF7 and T47D cells, under laminar flow, onto antibody-coated long alkylsilane self-assembled monolayers (SAMs) in a parallel plate flow chamber. The surface floor of the laminar flow chamber was grafted with an amino-terminated long alkyl chain spacer, 21-aminohenicosyl trichlorosilane (AHTS) followed by tethering a specific monoclonal antibody directed against the human epithelial cell adhesion molecule (EpCAM) antigen, which is overexpressed in primary breast cancer. Properties of the AHTS- and antibody-grafted surface floor were compared to that of surface floors coated with the short alkyl spacers 3-glycidoxy-propyl trimethoxysilane (GPTS) or 3-aminopropyl triethoxysilane (APTES) and antibodies. A theoretical model was constructed according to the geometry of the flow chamber in order to calculate the trajectories that would use cell flows. Cell capture experiments demonstrated that cell immobilization was optimized throughout the whole flow chamber. High cell capture was yielded on antibody-tethered long alkyl AHTS surface. This new procedure offers multiple advantages: a versatile tool readily applied to a panel of purified antibodies, an enrichment of cell immobilization using repetitive cell flow, and a stable capturing surface suitable for long term storage and handling.
|
Auteur(s) |
Jean-Claude Ehrhart
, Bernard Bennetau
1
, Louis Renaud
2
, Jean-Pierre Madrange
, Laurent Thomas
3
, Julie Morisot
4
, Arnaud Brosseau
5
, Sylvain Allano
, Patrick Tauc
6
, Phuong-Lan Tran
4
1
ISM -
Institut des Sciences Moléculaires
( 24493 )
- Bâtiment A 12 351 cours de la Libération 33405 TALENCE CEDEX
- France
2
CERCO -
Centre de recherche cerveau et cognition
( 722 )
- CHU Purpan - Pavillon Baudot BP 25202 31052 Toulouse Cedex
- France
3
Department of Physics
( 61767 )
- Royaume-Uni
4
SATIE -
Systèmes et Applications des Technologies de l'Information et de l'Energie
( 1194 )
- 61 av du President Wilson 94235 CACHAN Cedex
- France
5
PPSM -
Laboratoire de Photophysique et Photochimie Supramoléculaires et Macromoléculaires
( 1304 )
- bat. d'Alembert - RDC bas 61 Av du président Wilson 94235 CACHAN CEDEX
- France
6
LBPA -
Laboratoire de Biologie et de Pharmacologie Appliquée
( 1257 )
- 61 AVENUE DU PRESIDENT WILSON 94235 CACHAN CEDEX
- France
|
Comité de lecture |
Oui
|
Vulgarisation |
Non
|
Langue du document |
Anglais
|
Nom de la revue |
|
Audience |
Internationale
|
Date de publication |
2008-11-15
|
Date de publication électronique |
2008-05-08
|
Volume |
24
|
Numéro |
3
|
Page/Identifiant |
467-74
|
Domaine(s) |
|
Mots-clés (Mesh) |
|
DOI | 10.1016/j.bios.2008.04.027 |
Pubmed Id | 18547800 |
Loading...