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Article Dans Une Revue Bioorganic and Medicinal Chemistry Letters Année : 2009

Amino acid precursors for the detection of transketolase activity in Escherichia coli auxotrophs

Résumé

Probes were developed for the in vivo detection of transketolase activity by the use of a complementation assay in Escherichia coli auxotrophs They combine the d-threo ketose moiety recognised by transketolase and the side chain of leucine or methionine. These compounds were donor substrates of yeast transketolase leading to the release of the corresponding α-hydroxyaldehydes which could be converted in E. coli by a cascade of reactions into leucine or methionine required for cellular growth.

Dates et versions

hal-00413292 , version 1 (03-09-2009)

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Citer

Grégory Simon, Madeleine Bouzon, Franck Charmantray, Virgil Hélaine, Bertrand Legeret, et al.. Amino acid precursors for the detection of transketolase activity in Escherichia coli auxotrophs. Bioorganic and Medicinal Chemistry Letters, 2009, 19, pp.3767-3770. ⟨10.1016/j.bmcl.2009.04.111⟩. ⟨hal-00413292⟩
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