Fibrillarin and Nop56 interact before being co-assembled in box C/D snoRNPs. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Experimental Cell Research Année : 2009

Fibrillarin and Nop56 interact before being co-assembled in box C/D snoRNPs.

Résumé

Small nucleolar RNAs play crucial roles in ribosome biogenesis. They guide folding, site-specific nucleotide modifications and participate in cleavage of precursor ribosomal RNAs. To better understand how the biogenesis of the box C/D small nucleolar RNPs (snoRNPs) occur in a cellular context, we used a new approach based on the possibility of relocalizing a given nuclear complex by adding an affinity tag for B23 to one component of this complex. We selectively delocalized each core box C/D protein, namely 15.5kD, Nop56, Nop58 and fibrillarin, and analyzed the effect of such changes on other components of the box C/D snoRNPs. We show that modifying the localization and the mobility of core box C/D proteins impairs their association with box C/D snoRNPs. In addition, we demonstrate that fibrillarin and Nop56 directly interact in vivo. This interaction, indispensable for the association of both proteins with the box C/D snoRNPs, does not involve the glycine- and arginine-rich domain or the RNA-binding domain but the alpha-helix domain of fibrillarin. In addition, no RNA seems required to maintain fibrillarin-Nop56 interaction.

Dates et versions

hal-00394808 , version 1 (12-06-2009)

Identifiants

Citer

Tanguy Lechertier, Alice Grob, Danièle Hernandez-Verdun, Pascal Roussel. Fibrillarin and Nop56 interact before being co-assembled in box C/D snoRNPs.. Experimental Cell Research, 2009, 315 (6), pp.928-42. ⟨10.1016/j.yexcr.2009.01.016⟩. ⟨hal-00394808⟩
48 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More