Article Dans Une Revue Virus Research Année : 2009

Parainfluenza virus type 5 (PIV-5) morphology revealed by cryo-electron microscopy.

Résumé

The knowledge of parainfluenza type 5 (PIV-5) virion morphology is essentially based on the observation of negatively stained preparations in conventional transmission electron microscopy (CTEM). In this study, the ultrastructure of frozen-hydrated intact PIV-5 was examined by cryo-electron microscopy (cryo-EM). Cryo-EM revealed a majority of spherical virions (70%), with a lower pleiomorphy than originally observed in CTEM. Phospholipid bilayer thickness, spike length and glycoprotein spikes density were measured. About 2000 glycoprotein spikes were present in an average-sized spherical virion. Altogether, these data depict a more precise view of PIV-5 morphology.

Dates et versions

hal-00394452 , version 1 (11-06-2009)

Identifiants

Citer

Olivier Terrier, Jean-Paul Rolland, Manuel Rosa-Calatrava, Bruno Lina, Daniel Thomas, et al.. Parainfluenza virus type 5 (PIV-5) morphology revealed by cryo-electron microscopy.. Virus Research, 2009, 142 (1-2), pp.200-3. ⟨10.1016/j.virusres.2008.12.017⟩. ⟨hal-00394452⟩
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