Inhibition of transcription in Staphylococcus aureus by a primary sigma factor-binding polypeptide from phage G1. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Bacteriology Année : 2009

Inhibition of transcription in Staphylococcus aureus by a primary sigma factor-binding polypeptide from phage G1.

Mohammed Dehbi
  • Fonction : Auteur
Gregory Moeck
  • Fonction : Auteur
Francis F Arhin
  • Fonction : Auteur
Pascale Bauda
Dominique Bergeron
  • Fonction : Auteur
Tony Kwan
Jing Liu
John Mccarty
  • Fonction : Auteur
Jerry Pelletier
  • Fonction : Auteur

Résumé

The primary sigma factor of Staphylococcus aureus, sigma(SA), regulates transcription of many genes in this bacterium, including several essential genes, via specific recognition of exponential growth phase promoters. In this study, we report the existence of a novel staphylococcal phage G1-derived growth inhibitory polypeptide, referred to as G1ORF67, that interacts with sigma(SA) both in vivo and in vitro and regulates its activity. Delineation of the minimal domain of sigma(SA) that is required for its interaction with G1ORF67 to amino acids 294-360 near the carboxy terminus suggests that G1 phage-encoded anti-sigma factor may occlude the -35 element recognition domain of sigma(SA). As would be predicted by this hypothesis, the G1ORF67 polypeptide abolished both RNA polymerase core-dependent binding of sigma(SA) to DNA and sigma(SA)-dependent transcription in vitro. While G1ORF67 profoundly inhibits transcription when expressed in S. aureus cells in mode of action studies, our finding that G1ORF67 was unable to inhibit transcription when expressed in E. coli concurs with its inability to inhibit transcription in vitro by the Escherichia coli holoenzyme. These features demonstrate the selectivity of G1ORF67 for S. aureus RNA polymerase. We predict that G1ORF67 is one of the central polypeptides in the phage G1 strategy to appropriate host RNA polymerase and redirect it to phage reproduction.

Dates et versions

hal-00379075 , version 1 (27-04-2009)

Identifiants

Citer

Mohammed Dehbi, Gregory Moeck, Francis F Arhin, Pascale Bauda, Dominique Bergeron, et al.. Inhibition of transcription in Staphylococcus aureus by a primary sigma factor-binding polypeptide from phage G1.. Journal of Bacteriology, 2009, epub ahead of print. ⟨10.1128/JB.00241-09⟩. ⟨hal-00379075⟩
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