X-ray spectroscopy and X-ray diffraction at wavelengths near the K-absorption edge of phosphorus. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Synchrotron Radiation Année : 2005

X-ray spectroscopy and X-ray diffraction at wavelengths near the K-absorption edge of phosphorus.

Résumé

Phosphorus is an abundant element in living organisms. It is traceable by its X-ray absorption spectrum which shows a strong white line at its K-edge, comparable with that observed for the L(III) edges of rare earth ions. With purple membrane, the variation of the imaginary part of the anomalous dispersion of phosphorus is found to be close to 20 anomalous electron units. Anomalous diffraction experiments at wavelengths near the K-absorption edge of phosphorus confirm this result. The spatial distribution of lipids derived from anomalous diffraction agrees with earlier results from neutron diffraction. Test experiments on single crystals of the carrier protein using 5.76 A photons gave a first low-resolution diffraction pattern. Various techniques of crystal mounting were attempted. In addition, fluorescence measurements on a solution of threonine synthase appear to hint at a change of the phosphate environment of the cofactor upon activator binding.

Dates et versions

hal-00376039 , version 1 (16-04-2009)

Identifiants

Citer

Valérie Biou, Peter Bösecke, Jean Marie Bois, Gérard Brandolin, Richard Kahn, et al.. X-ray spectroscopy and X-ray diffraction at wavelengths near the K-absorption edge of phosphorus.. Journal of Synchrotron Radiation, 2005, 12 (Pt 4), pp.402-9. ⟨10.1107/S0909049505009222⟩. ⟨hal-00376039⟩
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