Titre Influence of polyelectrolyte coating conditions on capillary coating stability and separation efficiency in capillary electrophoresis Journal - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Electrophoresis Année : 2008

Titre Influence of polyelectrolyte coating conditions on capillary coating stability and separation efficiency in capillary electrophoresis Journal

Résumé

Polyelectrolytes are widely used in capillary electrophoresis as coating agents of silica capillaries to prevent adsorption phenomena and improve the repeatability of peptide and protein analysis. A systematic study of the coating experimental conditions has been carried out to optimize coating stability and performance. The main experimental parameters studied were the type and concentration of polyelectrolytes used in several monolayer and multilayer coatings, the ionic strength of coating and stabilizing solutions, and the procedures used for coating and capillary storage. Electroosmotic flow magnitude, direction and repeatability were used to monitor coating stability. Coating ability to limit adsorption was investigated by monitoring variations of migration times, time-corrected peak areas and separation efficiency of test peptides. Capillary-to-capillary and batch-to-batch reproducibility was also studied. In addition, the separation performance of polyelectrolyte coatings were compared to those obtained with bare silica capillaries.

Domaines

Chimie organique

Dates et versions

hal-00357945 , version 1 (02-02-2009)

Identifiants

Citer

R. Nehme, Catherine Perrin, Hervé Cottet, M.D. Blanchin, Huguette Fabre. Titre Influence of polyelectrolyte coating conditions on capillary coating stability and separation efficiency in capillary electrophoresis Journal. Electrophoresis, 2008, 29 (2), pp.3013-3023. ⟨10.1002/elps.200700886⟩. ⟨hal-00357945⟩
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