Research over the past year has outlined the importance of biogenic isoprene emission in tropospheric chemistry, and notably in the context of regional ozone photo-oxidant pollution. The first part of this article deals with the development of a simple isoprene emission scheme based upon the classical Guenther's algorithm coupled with a soil-vegetation-atmosphere transfer model. The resulting emission scheme is tested in a "stand-alone" version at the canopy scale. Experimental data sets coming from Boreal, Tropical, Temperate and Mediterranean ecosystems are used to estimate the robustness of the scheme over contrasted climatic and ecological conditions. Considering the simple hypothesis used, simulated isoprene fluxes are generally consistent with field measurements and the emission scheme is thus deemed suitable for regional application. Limitations of the model are outlined as well as further improvements. In the second part of the article, the emission scheme is used on line in the broader context of a meso-scale atmospheric chemistry scheme. Dynamically idealized simulations are carried out to study the chemical interactions of pollutant plumes with realistic isoprene emissions coming from a Mediterranean oak forest. Two chemical scenarios are considered with anthropogenic emissions, respectively representative of the Marseille (urban) and Martigues (industrial) French Mediterranean areas. For the Marseille scenario, the impact of biogenic emission on ozone production is larger when the forest is situated in a sub-urban configuration (i.e. downwind distance TOWN-FOREST <30 km) and decrease quite rapidly as the distance increases. For the Martigues scenario, the biogenic impact on the plume is detectable even at a longer TOWN-FOREST distance of 100 km. For both cases, the importance of the VOC/NO_x ratio, which characterizes the aging of advected pollutant plumes over the day, is outlined. Finally, possible applications of this work for real-case studies are discussed.