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Article Dans Une Revue PLoS ONE Année : 2008

Both functional LTbeta receptor and TNF receptor 2 are required for the development of experimental cerebral malaria.

Lizette Fick
  • Fonction : Auteur
Stefanie Scheu
  • Fonction : Auteur
Klaus Pfeffer
  • Fonction : Auteur
Robert Menard
  • Fonction : Auteur
Georges E Grau
  • Fonction : Auteur
Laurent Renia
  • Fonction : Auteur
Anna M Hansen
  • Fonction : Auteur
Helen J Ball
  • Fonction : Auteur
Nicholas H Hunt
  • Fonction : Auteur
Bernhard Ryffel
  • Fonction : Auteur
  • PersonId : 841831

Résumé

BACKGROUND: TNF-related lymphotoxin alpha (LTalpha) is essential for the development of Plasmodium berghei ANKA (PbA)-induced experimental cerebral malaria (ECM). The pathway involved has been attributed to TNFR2. Here we show a second arm of LTalpha-signaling essential for ECM development through LTbeta-R, receptor of LTalpha1beta2 heterotrimer. METHODOLOGY/PRINCIPAL FINDINGS: LTbetaR deficient mice did not develop the neurological signs seen in PbA induced ECM but died at three weeks with high parasitaemia and severe anemia like LTalphabeta deficient mice. Resistance of LTalphabeta or LTbetaR deficient mice correlated with unaltered cerebral microcirculation and absence of ischemia, as documented by magnetic resonance imaging and angiography, associated with lack of microvascular obstruction, while wild-type mice developed distinct microvascular pathology. Recruitment and activation of perforin(+) CD8(+) T cells, and their ICAM-1 expression were clearly attenuated in the brain of resistant mice. An essential contribution of LIGHT, another LTbetaR ligand, could be excluded, as LIGHT deficient mice rapidly succumbed to ECM. CONCLUSIONS/SIGNIFICANCE: LTbetaR expressed on radioresistant resident stromal, probably endothelial cells, rather than hematopoietic cells, are essential for the development of ECM, as assessed by hematopoietic reconstitution experiment. Therefore, the data suggest that both functional LTbetaR and TNFR2 signaling are required and non-redundant for the development of microvascular pathology resulting in fatal ECM.

Domaines

Immunologie

Dates et versions

hal-00318568 , version 1 (04-09-2008)

Identifiants

Citer

Dieudonnée Togbe, Paulo Loureiro de Sousa, Mathilde Fauconnier, Victorine Boissay, Lizette Fick, et al.. Both functional LTbeta receptor and TNF receptor 2 are required for the development of experimental cerebral malaria.. PLoS ONE, 2008, 3 (7), pp.e2608. ⟨10.1371/journal.pone.0002608⟩. ⟨hal-00318568⟩
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