Glycolipid-dependent sorting of melanosomal from lysosomal membrane proteins by lumenal determinants. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Traffic Année : 2008

Glycolipid-dependent sorting of melanosomal from lysosomal membrane proteins by lumenal determinants.

Résumé

Melanosomes are lysosome-related organelles that coexist with lysosomes in mammalian pigment cells. Melanosomal and lysosomal membrane proteins share similar sorting signals in their cytoplasmic tail, raising the question how they are segregated. We show that in control melanocytes, the melanosomal enzymes tyrosinase-related protein 1 (Tyrp1) and tyrosinase follow an intracellular Golgi to melanosome pathway, whereas in the absence of glycosphingolipids, they are observed to pass over the cell surface. Unexpectedly, the lysosome-associated membrane protein 1 (LAMP-1) and 2 behaved exactly opposite: they were found to travel through the cell surface in control melanocytes but followed an intracellular pathway in the absence of glycosphingolipids. Chimeric proteins having the cytoplasmic tail of Tyrp1 or tyrosinase were transported like lysosomal proteins, whereas a LAMP-1 construct containing the lumenal domain of Tyrp1 localized to melanosomes. In conclusion, the lumenal domain contains sorting information that guides Tyrp1 and probably tyrosinase to melanosomes by an intracellular route that excludes lysosomal proteins and requires glucosylceramide.

Dates et versions

hal-00318442 , version 1 (03-09-2008)

Identifiants

Citer

Sophie Groux-Degroote, Suzanne M van Dijk, Jasja Wolthoorn, Sylvia Neumann, Alexander C Theos, et al.. Glycolipid-dependent sorting of melanosomal from lysosomal membrane proteins by lumenal determinants.. Traffic, 2008, 9 (6), pp.951-63. ⟨10.1111/j.1600-0854.2008.00740.x⟩. ⟨hal-00318442⟩

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