Crystallization of the pectate lyase PelI from Erwinia chrysanthemi and SAD phasing of a gold derivative. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Acta Crystallogr D Biol Crystallogr Année : 2004

Crystallization of the pectate lyase PelI from Erwinia chrysanthemi and SAD phasing of a gold derivative.

Résumé

The pectate lyase PelI is involved in the degradation of plant tissues by the phytopathogenic bacterium Erwinia chrysanthemi. It has been crystallized from a solution containing PEG 550 in the space group P2(1), with unit-cell parameters a = 61.6, b = 70.7, c = 73.4 A, beta = 112.8 degrees. Crystals diffract to 1.45 A using synchrotron radiation. SAD phases have been computed from a gold-derivative crystal at the wavelength of maximum absorption (L(III) edge).The pectate lyase PelI is involved in the degradation of plant tissues by the phytopathogenic bacterium Erwinia chrysanthemi. It has been crystallized from a solution containing PEG 550 in the space group P2(1), with unit-cell parameters a = 61.6, b = 70.7, c = 73.4 A, beta = 112.8 degrees. Crystals diffract to 1.45 A using synchrotron radiation. SAD phases have been computed from a gold-derivative crystal at the wavelength of maximum absorption (L(III) edge).
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Dates et versions

hal-00313538 , version 1 (27-08-2008)

Identifiants

  • HAL Id : hal-00313538 , version 1

Citer

S. Castang, Ve Shevchik, N. Hugouvieux-Cotte-Pattat, P. Legrand, R. Haser, et al.. Crystallization of the pectate lyase PelI from Erwinia chrysanthemi and SAD phasing of a gold derivative.. Acta Crystallogr D Biol Crystallogr, 2004, 60, pp.190-192. ⟨hal-00313538⟩
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