Theoretical study of ethidium intercalation in triple-stranded DNA and at triplex-duplex junctions. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biomolecular Structure and Dynamics Année : 1991

Theoretical study of ethidium intercalation in triple-stranded DNA and at triplex-duplex junctions.

Résumé

The contribution of different factors in the interaction of ethidium intercalated into various sequences of a triple helix, or in the region of the junction between the double- and triple-stranded DNA has been studied by energy minimization. It is found that in the total energy of the ethidium- triple helix complexes, a particular electrostatic contribution emerges due to the presence of protonated cytosines in the triple helix. This parameters is determinant in the sequence-specificity of ethidium binding to the triple helix. The preferred intercalation sites of ethidium in the triple helix are proposed. The interaction of ethidium at the triplex-duplex junction, and its effects are also discussed. This study is aimed at searching for new drugs specific for the triple helix, or for the triplex-duplex junctions.The contribution of different factors in the interaction of ethidium intercalated into various sequences of a triple helix, or in the region of the junction between the double- and triple-stranded DNA has been studied by energy minimization. It is found that in the total energy of the ethidium- triple helix complexes, a particular electrostatic contribution emerges due to the presence of protonated cytosines in the triple helix. This parameters is determinant in the sequence-specificity of ethidium binding to the triple helix. The preferred intercalation sites of ethidium in the triple helix are proposed. The interaction of ethidium at the triplex-duplex junction, and its effects are also discussed. This study is aimed at searching for new drugs specific for the triple helix, or for the triplex-duplex junctions.
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Dates et versions

hal-00312972 , version 1 (27-08-2008)

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  • HAL Id : hal-00312972 , version 1

Citer

Js Sun, R. Lavery, J. Chomilier, K. Zakrzewska, T. Montenay-Garestier, et al.. Theoretical study of ethidium intercalation in triple-stranded DNA and at triplex-duplex junctions.. Journal of Biomolecular Structure and Dynamics, 1991, 9, pp.425-436. ⟨hal-00312972⟩
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