Ca2+ homeostasis regulates Xenopus oocyte maturation. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biology of Reproduction Année : 2008

Ca2+ homeostasis regulates Xenopus oocyte maturation.

Lu Sun
  • Fonction : Auteur
Rawad Hodeify
  • Fonction : Auteur
Shirley Haun
  • Fonction : Auteur
Amanda Charlesworth
  • Fonction : Auteur
Angus M Macnicol
  • Fonction : Auteur
Subramaniam Ponnappan
  • Fonction : Auteur
Usha Ponnappan
  • Fonction : Auteur
Khaled Machaca
  • Fonction : Auteur

Résumé

In contrast to the well-defined role of Ca2+ signals during mitosis, the contribution of Ca2+ signaling to meiosis progression is controversial, despite several decades of investigating the role of Ca2+ and its effectors in vertebrate oocyte maturation. We have previously shown that during Xenopus oocyte maturation, Ca2+ signals are dispensable for entry into meiosis and for germinal vesicle breakdown. However, normal Ca2+ homeostasis is essential for completion of meiosis I and extrusion of the first polar body. In this study, we test the contribution of several downstream effectors in mediating the Ca2+ effects during oocyte maturation. We show that calmodulin and calcium-calmodulin-dependent protein kinase II (CAMK2) are not critical downstream Ca2+ effectors during meiotic maturation. In contrast, accumulation of Aurora kinase A (AURKA) protein is disrupted in cells deprived of Ca2+ signals. Since AURKA is required for bipolar spindle formation, failure to accumulate AURKA may contribute to the defective spindle phenotype following Ca2+ deprivation. These findings argue that Ca2+ homeostasis is important in establishing the oocyte's competence to undergo maturation in preparation for fertilization and embryonic development.

Dates et versions

hal-00277220 , version 1 (05-05-2008)

Identifiants

Citer

Lu Sun, Rawad Hodeify, Shirley Haun, Amanda Charlesworth, Angus M Macnicol, et al.. Ca2+ homeostasis regulates Xenopus oocyte maturation.. Biology of Reproduction, 2008, 78 (4), pp.726-35. ⟨10.1095/biolreprod.107.063693⟩. ⟨hal-00277220⟩
79 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More