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Article Dans Une Revue Methods Année : 2007

Fluorescence-based melting assays for studying quadruplex ligands.

Résumé

The telomeric G-rich single-stranded DNA can adopt in vitro an intramolecular quadruplex structure, which has been shown to directly inhibit telomerase activity. The reactivation of this enzyme in immortalized and most cancer cells suggests that telomeres and telomerase are relevant targets in oncology, and telomere ligands and telomerase inhibitors have been proposed as new potential anticancer agents. In this paper, we have analysed the FRET method used to measure the stabilization and selectivity of quadruplex ligands towards the human telomeric G-quadruplex. The stabilization value depends on the nature of the fluorescent tags, the incubation buffer, and the method chosen for T(m) calculation, complicating a direct comparison of the results obtained by different laboratories.

Domaines

Chimie organique

Dates et versions

hal-00258193 , version 1 (21-02-2008)

Identifiants

Citer

Anne de Cian, Lionel Guittat, Markus Kaiser, Barbara Saccà, Samir Amrane, et al.. Fluorescence-based melting assays for studying quadruplex ligands.. Methods, 2007, 42 (2), pp.183-95. ⟨10.1016/j.ymeth.2006.10.004⟩. ⟨hal-00258193⟩

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