Differentiated rabbit prostatic stromal cells in primary culture display functional alpha1A-adrenoceptors. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Neurourology and Urodynamics Année : 2006

Differentiated rabbit prostatic stromal cells in primary culture display functional alpha1A-adrenoceptors.

Delphine Boulbès
  • Fonction : Auteur
Armelle Choquet
  • Fonction : Auteur
Stéphanie Barrère-Lemaire
Pierre Costa
  • Fonction : Auteur
Laurent Soustelle
  • Fonction : Auteur
Joël Nargeot
  • Fonction : Auteur
Jean-Pierre Bali
  • Fonction : Auteur
Frédéric Hollande

Résumé

AIMS: BPH is characterized by uncontrolled proliferation and increased contractility of prostatic smooth muscle cells. The activation of alpha1-adrenoceptors (alpha1-AR) seems involved in the latter event, but the lack of in vitro models expressing these receptors has hampered a more specific characterization of their role. In order to do so, we attempted to develop a new model of rabbit cultured prostatic stromal cells (PSC) in a non-proliferative and differentiated state. METHODS: The expression of cytoskeletal and stromal markers was confirmed by immunohistochemistry on primary cultured PSC. Alpha1-AR subtype expression was assessed by RT-PCR, while receptor coupling to the ERK1/ERK2 and calcium pathways was studied by Western Blot and Fura-2 calcium imaging, respectively. RESULTS: Cells grown under non-proliferative conditions displayed a differentiated phenotype, with expression of contractile cytoskeletal and stromal proteins. Furthermore, the alpha1A-AR was shown to activate ERK1/ERK2 as well as calcium signaling. CONCLUSION: These results emphasize the interest of this model for the characterization of PSC adrenergic regulation, in particular through the little-known alpha1A-AR.

Dates et versions

hal-00201272 , version 1 (27-12-2007)

Identifiants

Citer

Delphine Boulbès, Armelle Choquet, Stéphanie Barrère-Lemaire, Pierre Costa, Laurent Soustelle, et al.. Differentiated rabbit prostatic stromal cells in primary culture display functional alpha1A-adrenoceptors.. Neurourology and Urodynamics, 2006, 25 (2), pp.168-78. ⟨10.1002/nau.20196⟩. ⟨hal-00201272⟩
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