DNA ejection from bacteriophage: towards a general behavior for osmotic suppression experiments - Archive ouverte HAL
Article Dans Une Revue European Physical Journal E: Soft matter and biological physics Année : 2007

DNA ejection from bacteriophage: towards a general behavior for osmotic suppression experiments

Résumé

We present in this work in vitro measurements of the force ejecting DNA from two distinct bacteriophages (T5 and lambda) using the smotic-suppression technique. Our data are analyzed by revisiting the current theories of DNA packaging in spherical capsids. In particular we show that a simplified analytical model based on bending considerations only is able to account quantitatively for the experimental findings. Physical and biological consequences are discussed.
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Dates et versions

hal-00196724 , version 1 (13-12-2007)

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Martin Castelnovo, Alex Evilevitch. DNA ejection from bacteriophage: towards a general behavior for osmotic suppression experiments. European Physical Journal E: Soft matter and biological physics, 2007, 24, pp.9-18. ⟨10.1140/epje/i2007-10205-5⟩. ⟨hal-00196724⟩
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