Crustacean hyperglycemic and vitellogenesis-inhibiting hormones in the lobster Homarus gammarus. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEBS Journal Année : 2006

Crustacean hyperglycemic and vitellogenesis-inhibiting hormones in the lobster Homarus gammarus.

Résumé

Crustacean hyperglycemic hormone (CHH) and vitellogenesis-inhibiting hormone (VIH), produced by the X organ-sinus gland neurosecretory complex, belong to a peptide group referred to as the CHH family, which is widely distributed in arthropods. In this study, genetic variants and post-translationally modified isoforms of CHH and VIH were characterized in the European lobster Homarus gammarus. With the use of RP-HPLC and ELISA with specific antibodies that discriminate between stereoisomers of CHH and VIH, two groups of CHH-immunoreactive peaks were characterized from HPLC fractions of sinus gland extract (CHH A and CHH B); each group contained two variants (CHH and D-Phe3CHH). In the same way, two VIH-immunoreactive peaks (VIH and D-Trp4VIH) were demonstrated in HPLC fractions from sinus gland extract. The masses of these different neuropeptides were determined by FT-ICR MS: CHH A and CHH B spectra exhibited monoisotopic ions at 8557.05 Da and 8527.04 Da, respectively, and both VIH isomers displayed an m/z value of 9129.19 Da. Two full-length cDNAs encoding preprohomones of CHH A and CHH B and only one cDNA for VIH precursor were cloned and sequenced from X organ RNA. Comparison of CHH sequences between European lobster and other Astacoidea suggests that the most hydrophobic form appeared first during crustacean evolution.
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Dates et versions

hal-00196118 , version 1 (12-12-2007)

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Céline Ollivaux, Joelle Vinh, Daniel Soyez, Jean-Yves Toullec. Crustacean hyperglycemic and vitellogenesis-inhibiting hormones in the lobster Homarus gammarus.. FEBS Journal, 2006, 273 (10), pp.2151-60. ⟨10.1111/j.1742-4658.2006.05228.x⟩. ⟨hal-00196118⟩
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